Overview

  • Product name

    Anti-Elp4 antibody [EPR4200(2)]
    See all Elp4 primary antibodies
  • Description

    Rabbit monoclonal [EPR4200(2)] to Elp4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Elp4 aa 300-400 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Human stomach tissue; HepG2 cells; 293T, NIH/3T3, Y79, U87-MG and HeLa whole cell lysate (ab150035).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the ELP4 family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C11orf19 antibody
      • dJ68P15A.1 antibody
      • elongation protein 4 homolog (S. cerevisiae) antibody
      • Elongation protein 4 homolog antibody
      • Elongator complex protein 4 antibody
      • ELP4 antibody
      • ELP4_HUMAN antibody
      • FLJ20498 antibody
      • hELP4 antibody
      • PAX6 neighbor gene protein antibody
      • PAX6NEB antibody
      • PAXNEB antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Elp4 with ab133687 at 1/250 dilution.
    • Immunofluorescent analysis of HepG2 cells labelling Elp4 with ab133687 at 1/250 dilution.
    • All lanes : Anti-Elp4 antibody [EPR4200(2)] (ab133687) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : NIH/3T3 cell lysate
      Lane 3 : Y79 cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 46 kDa

    References

    ab133687 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133687.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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