Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4200(2)] to Elp4
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Elp4 antibody [EPR4200(2)]
See all Elp4 primary antibodies
DescriptionRabbit monoclonal [EPR4200(2)] to Elp4
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Elp4 aa 300-400 (internal sequence). The exact sequence is proprietary.
- Human stomach tissue; HepG2 cells; 293T, NIH/3T3, Y79, U87-MG and HeLa whole cell lysate (ab150035).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- HeLa nuclear extract lysate (ab14655)
- NIH 3T3 cytoplasmic extract lysate (ab14873)
- NIH 3T3 nuclear extract lysate (ab14874)
- U87 MG nuclear extract lysate (ab14903)
- Y79 nuclear extract lysate (ab14915)
- HeLa whole cell lysate (ab29545)
- NIH 3T3 whole cell lysate (ab7179)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab133687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 46 kDa.|
FunctionActs as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the ELP4 family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- C11orf19 antibody
- dJ68P15A.1 antibody
- elongation protein 4 homolog (S. cerevisiae) antibody
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Elp4 with ab133687 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of HepG2 cells labelling Elp4 with ab133687 at 1/250 dilution.
All lanes : Anti-Elp4 antibody [EPR4200(2)] (ab133687) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : Y79 cell lysate
Lane 4 : U87-MG cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
ab133687 has been referenced in 1 publication.
- Li MT et al. ELP3 Acetyltransferase is phosphorylated and regulated by the oncogenic anaplastic lymphoma kinase (ALK). Biochem J 476:2239-2254 (2019). PubMed: 31341009