Product nameAnti-Elp4 antibody [EPR4200(2)]
See all Elp4 primary antibodies
DescriptionRabbit monoclonal [EPR4200(2)] to Elp4
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Elp4 aa 300-400 (internal sequence). The exact sequence is proprietary.
- Human stomach tissue; HepG2 cells; 293T, NIH/3T3, Y79, U87-MG and HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- HeLa nuclear extract lysate (ab14655)
- NIH 3T3 cytoplasmic extract lysate (ab14873)
- NIH 3T3 nuclear extract lysate (ab14874)
- U87 MG nuclear extract lysate (ab14903)
- Y79 nuclear extract lysate (ab14915)
- HeLa whole cell lysate (ab29545)
- NIH 3T3 whole cell lysate (ab7179)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab133687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 46 kDa.|
FunctionActs as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the ELP4 family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- C11orf19 antibody
- dJ68P15A.1 antibody
- elongation protein 4 homolog (S. cerevisiae) antibody
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Elp4 with ab133687 at 1/250 dilution.
Immunofluorescent analysis of HepG2 cells labelling Elp4 with ab133687 at 1/250 dilution.
All lanes : Anti-Elp4 antibody [EPR4200(2)] (ab133687) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : Y79 cell lysate
Lane 4 : U87-MG cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
ab133687 has not yet been referenced specifically in any publications.