Product nameAnti-ELYS antibody
See all ELYS primary antibodies
DescriptionRabbit polyclonal to ELYS
SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa2220 and aa2302 of human ELYS using the numbering given in entry AAN65622.1 (GeneID 25909).
- HeLa cells nuclear extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 8% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified using an epitope specific to ELYS immobilized on solid support.
Our Abpromise guarantee covers the use of ab14431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 272 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Prepare working dilution immediately before use.
FunctionRequired for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.
Sequence similaritiesBelongs to the ELYS family.
Contains 1 A.T hook DNA-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus matrix. Chromosome > centromere > kinetochore. Nucleus > nucleoplasm. Nucleus > nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- ahctf1 antibody
- AT hook containing transcription factor 1 antibody
- DKFZp434N093 antibody
All lanes : Anti-ELYS antibody (ab14431) at 0.5 µg/ml
Lane 1 : Cell lysates prepared from Hela cells at 10 µg/ml
Lane 2 : Cell lysates prepared from Hela cells at 20 µg/ml
Predicted band size: 272 kDa
ab14431 at 0.5mg/ml staining human ELYS by Western blot using chemiluminescence with exposure times of 3-5 minutes. Left lane 10mg HeLa nuclear extract, right lane 20mg.
ab14431 has not yet been referenced specifically in any publications.