• Product name
    Anti-EMAP II antibody [546-2]
    See all EMAP II primary antibodies
  • Description
    Mouse monoclonal [546-2] to EMAP II
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant human EMAP II



Our Abpromise guarantee covers the use of ab15693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/40.
IHC-P 1/40. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 34 kDa.


  • Function
    Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • Involvement in disease
    Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
  • Sequence similarities
    Contains 1 tRNA-binding domain.
  • Post-translational
    Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIMP 1 antibody
    • Aimp1 antibody
    • AIMP1/p43 antibody
    • AIMP1_HUMAN antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody
    • ARS interacting multifunctional protein 1 antibody
    • EMAP 2 antibody
    • EMAP-2 antibody
    • EMAP-II antibody
    • EMAP2 antibody
    • EMAPII antibody
    • Endothelial monocyte activating polypeptide 2 antibody
    • Endothelial monocyte activating polypeptide antibody
    • Endothelial monocyte-activating polypeptide 2 antibody
    • Endothelial monocyte-activating polypeptide II antibody
    • HLD3 antibody
    • Multisynthase complex auxiliary component p43 antibody
    • Multisynthetase complex auxiliary component p43 antibody
    • OTTHUMP00000219673 antibody
    • p43 antibody
    • Scye 1 antibody
    • Scye1 antibody
    • Scye1, formerly antibody
    • Small inducible cytokine subfamily E member 1 antibody
    • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
    • Small inducible cytokine subfamily E, member 1, formerly antibody
    see all


  • Lanes 1-4 & 6-9 : Anti-EMAP II antibody [546-2] (ab15693) at 1/1000 dilution

    Lane 1 : HeLa Cytoplasmic Extract (0.625ug)
    Lane 2 : HeLa Cytoplasmic Extract (1.25ug)
    Lane 3 : HeLa Cytoplasmic Extract (2.5ug)
    Lane 4 : HeLa Cytoplasmic Extract (5ug)
    Lanes 5 & 10 : High Range Rainbow MWMs
    Lane 6 : HeLa Nuclear Extract (0.625ug)
    Lane 7 : HeLa Nuclear Extract (1.25ug)
    Lane 8 : HeLa Nuclear Extract (2.5ug)
    Lane 9 : HeLa Nuclear Extract (5ug)

    Lanes 1-4 & 6-9 : HRP conjugated Sheep anti-mouse IgG

    Predicted band size: 34 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible non-specific binding), 66 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)

    Samples were run on a 4-12% polyacrylamide gradient gel (SDS, Bis-Tris). Blocking and secondary antibody incubations were at room temperature. Primary antibody incubation was at 4°C.Primary and secondary antibodies were diluted in TBST containing 5% milk.

    The predominant band observed was 43 kDa, consistent with the expected mobility of pro-EmapII/p43.  The 21kDa "cleaved" form, EmapII, was not observed most likely because the cells were not treated to induce cleavage of pro-EmapII to release EmapII.  The identity of the 50kDa "nonspecific" band is not known, but it does not appear as prominent when a different EmapII antibody (ab10605) is used.

    See Abreview


This product has been referenced in:
  • Pechenino AS  et al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB ; Rat . Read more (PubMed: 21750230) »
  • Marik C  et al. Lesion genesis in a subset of patients with multiple sclerosis: a role for innate immunity? Brain 130:2800-15 (2007). ICC/IF . Read more (PubMed: 17956913) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Rat Tissue lysate - whole (Heart--Left ventricle)
Loading amount
40 µg
Heart--Left ventricle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Angela Pechenino

Verified customer

Submitted Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - other (HeLa Cytoplasmic Extract; HeLa Nuclear Extract)
Loading amount
5 µg
HeLa Cytoplasmic Extract; HeLa Nuclear Extract
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Nov 16 2006


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