Overview

  • Product name

    Anti-EMAP II/AIMP1 antibody
    See all EMAP II/AIMP1 primary antibodies
  • Description

    Goat polyclonal to EMAP II/AIMP1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human EMAP II/AIMP1 aa 137-149 (internal sequence) (Cysteine residue).
    Sequence:

    KQQSIAGSADSKP

  • Positive control

    • Human lymph node and Uterus lysates
  • General notes

     This product was previously labelled as EMAP II

     

Properties

Applications

Our Abpromise guarantee covers the use of ab139118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • Involvement in disease

    Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
  • Sequence similarities

    Contains 1 tRNA-binding domain.
  • Post-translational
    modifications

    Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
  • Information by UniProt
  • Database links

  • Alternative names

    • AIMP 1 antibody
    • Aimp1 antibody
    • AIMP1/p43 antibody
    • AIMP1_HUMAN antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody
    • ARS interacting multifunctional protein 1 antibody
    • EMAP 2 antibody
    • EMAP-2 antibody
    • EMAP-II antibody
    • EMAP2 antibody
    • EMAPII antibody
    • Endothelial monocyte activating polypeptide 2 antibody
    • Endothelial monocyte activating polypeptide antibody
    • Endothelial monocyte-activating polypeptide 2 antibody
    • Endothelial monocyte-activating polypeptide II antibody
    • HLD3 antibody
    • Multisynthase complex auxiliary component p43 antibody
    • Multisynthetase complex auxiliary component p43 antibody
    • OTTHUMP00000219673 antibody
    • p43 antibody
    • Scye 1 antibody
    • Scye1 antibody
    • Scye1, formerly antibody
    • Small inducible cytokine subfamily E member 1 antibody
    • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
    • Small inducible cytokine subfamily E, member 1, formerly antibody
    see all

Images

  • ab139118 (5µg/ml) staining of paraffin embedded Human Pancreas following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining in the cytoplasm towards the alpha cells periphery.

  • Anti-EMAP II/AIMP1 antibody (ab139118) at 0.1 µg/ml + Human lymph node cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 34 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab139118 has not yet been referenced specifically in any publications.

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