Key features and details
- Goat polyclonal to EMAP II/AIMP1 (Biotin)
- Suitable for: ELISA, Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-EMAP II/AIMP1 antibody (Biotin)
See all EMAP II/AIMP1 primary antibodies
DescriptionGoat polyclonal to EMAP II/AIMP1 (Biotin)
Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human EMAP II/AIMP1.
General notesab83518 is sterile filtered.
This product was previously labelled as EMAP II
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 34 kDa.|
FunctionNon-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
Involvement in diseaseDefects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
Sequence similaritiesContains 1 tRNA-binding domain.
modificationsCleaved by caspase-7 in response to apoptosis to produce EMAP-II.
Cellular localizationNucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
- Information by UniProt
- AIMP 1 antibody
- Aimp1 antibody
- AIMP1/p43 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab83518 has not yet been referenced specifically in any publications.