Overview

  • Product name

    Anti-EMAP II/AIMP1 antibody - N-terminal
    See all EMAP II/AIMP1 primary antibodies
  • Description

    Rabbit polyclonal to EMAP II/AIMP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human EMAP II/AIMP1 aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: Q12904

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as EMAP II

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
    • Involvement in disease

      Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
    • Sequence similarities

      Contains 1 tRNA-binding domain.
    • Post-translational
      modifications

      Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
    • Cellular localization

      Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
    • Information by UniProt
    • Database links

    • Alternative names

      • AIMP 1 antibody
      • Aimp1 antibody
      • AIMP1/p43 antibody
      • AIMP1_HUMAN antibody
      • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody
      • ARS interacting multifunctional protein 1 antibody
      • EMAP 2 antibody
      • EMAP-2 antibody
      • EMAP-II antibody
      • EMAP2 antibody
      • EMAPII antibody
      • Endothelial monocyte activating polypeptide 2 antibody
      • Endothelial monocyte activating polypeptide antibody
      • Endothelial monocyte-activating polypeptide 2 antibody
      • Endothelial monocyte-activating polypeptide II antibody
      • HLD3 antibody
      • Multisynthase complex auxiliary component p43 antibody
      • Multisynthetase complex auxiliary component p43 antibody
      • OTTHUMP00000219673 antibody
      • p43 antibody
      • Scye 1 antibody
      • Scye1 antibody
      • Scye1, formerly antibody
      • Small inducible cytokine subfamily E member 1 antibody
      • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
      • Small inducible cytokine subfamily E, member 1, formerly antibody
      see all

    Images

    • EMAP II/AIMP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226337 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226337 at 1 µg/ml.

      Lane 1: ab226337 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab226337 has not yet been referenced specifically in any publications.

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