Product nameAnti-Embigin homolog antibody
See all Embigin homolog primary antibodies
DescriptionRabbit polyclonal to Embigin homolog
Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 122-315 of Human Embigin homolog (UniProt Q6PCB8).
- 293T, A431, Jurkat and Raji whole cell lysates; A431 cells This antibody gave a positive result in IHC in the following FFPE tissue: Human Kidney.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab127692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionMay function as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues.
Sequence similaritiesContains 2 Ig-like V-type (immunoglobulin-like) domains.
- Information by UniProt
- Emb antibody
- EMB_HUMAN antibody
- Embigin antibody
IHC image of Embigin Homolog staining in Human Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab127692, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Embigin homolog antibody (ab127692) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS PAGE
ab127692, at a dilution of 1/500, staining Embigin homolog in methanol-fixed A431 cells by Immunofluorescence.
The lower image was co-stained with Hoechst 33342.
ab127692 has not yet been referenced specifically in any publications.