Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156)

Overview

  • Product name

    Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1)
  • Product overview

    ab107156 is a Mouse Embryonic Stem Cell Marker Panel containing four antibodies: 50µg of Oct4 rabbit polyclonal, 50µl of Nanog rabbit polyclonal, 50µg of SOX2 rabbit polyclonal and 50µl of SSEA1 mouse monoclonal.


    The Mouse Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived mouse embryonic stem (ES) cell lines. The panel contains four antibodies to well known markers of mouse ES cells (Oct4, Nanog, SOX2 and SSEA1). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).


    The antibodies in this panel were selected for their exceptional performance in ICC/IF in mouse cells. In addition, these antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information. Recommended working dilutions for ICC/IF in mouse can also be found in the figure legends on this datasheet.

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Properties

Images

  • ICC/IF image of Oct4 (ab19857, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in 4% paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Oct4 (1:250) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1:500 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue).
  • ab80892 staining Nanog (green) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1:200 and incubated with the sample overnight at 4°C. An Alexa Fluor conjugated goat anti rabbit IgG antibody, diluted 1:500, was used as the secondary and incubated with samples for 1 hour at room temperature.
  • ICC/IF image of Sox2 (ab97959, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Sox2 (1:500) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1:500 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue).
  • ab16285 staining SSEA1 (red) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1:10 and incubated with the sample overnight at 4°C. An Alexa Fluor 647 conjugated goat anti mouse IgM antibody, diluted 1:500, was used as the secondary and incubated with samples for 1 hour at room temperature.

References

This product has been referenced in:

  • Yang L  et al. KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system. EMBO Rep 19:N/A (2018). Read more (PubMed: 30389724) »
  • Kuan II  et al. EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2a. Sci Rep 7:41852 (2017). Read more (PubMed: 28157205) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

Based on other images for this product and other SOX2 antibodies, this staining pattern does look correct. However, it seems that there is room for improvement with this staining. Do you permeablize your tissues? Do you block in serum? I did see that one of the Abreviews for this product achieved success using glycine blocking. That same review also used an antigen retrieval, which while uncommon in IHC-Fr, can help with any paraformaldehyde fixation. Have you had the opportunity to run a series dilution with the antibody to determine the best concentration in this protocol? Do your washes contain a mild detergent? I would be very happy to review the protocol which you have used and offer suggestions if you provide that to me.

I hope this information is helpful to you and look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you very much for your email.

I am sorry we unfortunately do not provide services that includes testing of our customers sample. We provide guarantee with products, which states if the products does not work as stated on the datasheet we will give full money back.



I hope this information is nevertheless helpful to you. Should you have any further question please do not hesitate to contact me.

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Answer

Thank you for contacting us.

Could you please clarify, what you mean by service?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you very much for your interest in our antibodies and kits.

As discussed on the telephone, to our knowledge, ab2426 PCNA antibody,ab97959 SOX2 antibody and ab107156 Mouse Embryonic Stem Cell Marker Panel have not been testedand guaranteed for shark samples.Therefore, I can offer a discount off a future purchase if you buy ab2426 PCNA antibody and/or ab97959SOX2 antibody now, test it in shark and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. I am sorry we are not able to offer anything for the ab107156 panel on this occasion.

If you are interested in this offer for ab2426 and ab97959, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab2426 and/or ab97959 in shark. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

2. Purchase ab2426 and/or ab97959 either by phone, fax, or online (www.abcam.com).

3. Test it in shark.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2426 and/or ab97959 turns out to be unsuitable for shark, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at:
https://www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

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