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  1. Link

    emc4-antibody-epr15080-ab184544.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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RecombinantRabMAb

Recombinant Anti-EMC4 antibody [EPR15080] (ab184544)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (4)

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Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Anti-EMC4 antibody [EPR15080] (ab184544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15080] to EMC4
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Human

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Carrier Free

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Overview

  • Product name

    Anti-EMC4 antibody [EPR15080]
    See all EMC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR15080] to EMC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, SH-SY5Y, U87-MG and HepG2 cell lysates; Human brain and ovarian carcinoma tissues; mouse liver tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15080
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Associated products

  • Alternative Versions

    • Anti-EMC4 antibody [EPR15080] - BSA and Azide free (ab236141)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/50 - 1/70.
WB
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IP
1/50 - 1/70.
WB
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May mediate anti-apoptotic activity.
  • Tissue specificity

    Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart.
  • Sequence similarities

    Belongs to the TMEM85 family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q5J8M3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51234 Human
    • Entrez Gene: 68032 Mouse
    • Entrez Gene: 296049 Rat
    • SwissProt: Q5J8M3 Human
    • SwissProt: Q9CZX9 Mouse
    • Unigene: 250905 Human
    • Alternative names

      • Cell proliferation-inducing gene 17 protein antibody
      • EMC4 antibody
      • ER membrane protein complex subunit 4 antibody
      • HSPC184 antibody
      • PIG17 antibody
      • tmem85 antibody
      • TMM85_HUMAN antibody
      • Transmembrane protein 85 antibody
      see all

    Images

    • Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
      Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
      All lanes : Anti-EMC4 antibody [EPR15080] (ab184544) at 1/10000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 20 kDa
      Observed band size: 17,20 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

      Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)
      Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)

      Western blot analysis of EMC4 in HepG2 cell lysate immunoprecipitated using ab184544 at 1/50 dilution (Lane 1). Lane 2: PBS.

      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Anti-EMC4 antibody [EPR15080] (ab184544)
      Anti-EMC4 antibody [EPR15080] (ab184544)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (4)

    Publishing research using ab184544? Please let us know so that we can cite the reference in this datasheet.

    ab184544 has been referenced in 4 publications.

    • Campos RK  et al. Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins. Nucleic Acids Res 48:9872-9885 (2020). PubMed: 32890404
    • Miller-Vedam LE  et al. Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients. Elife 9:N/A (2020). PubMed: 33236988
    • Coelho JPL  et al. A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration. Nat Commun 10:672 (2019). PubMed: 30737405
    • Tian S  et al. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep 28:2517-2526.e5 (2019). PubMed: 31484065

    Customer reviews and Q&As

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