Recombinant Anti-EMC4 antibody [EPR15080] (ab184544)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15080] to EMC4
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-EMC4 antibody [EPR15080]
See all EMC4 primary antibodies -
Description
Rabbit monoclonal [EPR15080] to EMC4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- 293, SH-SY5Y, U87-MG and HepG2 cell lysates; Human brain and ovarian carcinoma tissues; mouse liver tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15080 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/50 - 1/70.
|
|
WB |
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa).
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
IP
1/50 - 1/70. |
WB
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
May mediate anti-apoptotic activity. -
Tissue specificity
Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart. -
Sequence similarities
Belongs to the TMEM85 family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 51234 Human
- Entrez Gene: 68032 Mouse
- Entrez Gene: 296049 Rat
- SwissProt: Q5J8M3 Human
- SwissProt: Q9CZX9 Mouse
- Unigene: 250905 Human
-
Alternative names
- Cell proliferation-inducing gene 17 protein antibody
- EMC4 antibody
- ER membrane protein complex subunit 4 antibody
see all
Images
-
All lanes : Anti-EMC4 antibody [EPR15080] (ab184544) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 17,20 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Western blot analysis of EMC4 in HepG2 cell lysate immunoprecipitated using ab184544 at 1/50 dilution (Lane 1). Lane 2: PBS.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (4)
ab184544 has been referenced in 4 publications.
- Campos RK et al. Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins. Nucleic Acids Res 48:9872-9885 (2020). PubMed: 32890404
- Miller-Vedam LE et al. Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients. Elife 9:N/A (2020). PubMed: 33236988
- Coelho JPL et al. A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration. Nat Commun 10:672 (2019). PubMed: 30737405
- Tian S et al. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep 28:2517-2526.e5 (2019). PubMed: 31484065