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RecombinantRabMAb

Recombinant Anti-EMC4 antibody [EPR15080] (ab184544)

Submit a review Submit a question References (4)
Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Anti-EMC4 antibody [EPR15080] (ab184544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15080] to EMC4
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-EMC4 antibody [EPR15080]
    See all EMC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR15080] to EMC4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, SH-SY5Y, U87-MG and HepG2 cell lysates; Human brain and ovarian carcinoma tissues; mouse liver tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15080

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/50 - 1/70.
WB
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IP
1/50 - 1/70.
WB
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
    Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
    All lanes : Anti-EMC4 antibody [EPR15080] (ab184544) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 17,20 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)

    Western blot analysis of EMC4 in HepG2 cell lysate immunoprecipitated using ab184544 at 1/50 dilution (Lane 1). Lane 2: PBS.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-EMC4 antibody [EPR15080] (ab184544)
    Anti-EMC4 antibody [EPR15080] (ab184544)

References (4)

Publishing research using ab184544? Please let us know so that we can cite the reference in this datasheet.

ab184544 has been referenced in 4 publications.

  • Campos RK  et al. Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins. Nucleic Acids Res 48:9872-9885 (2020). PubMed: 32890404
  • Miller-Vedam LE  et al. Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients. Elife 9:N/A (2020). PubMed: 33236988
  • Coelho JPL  et al. A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration. Nat Commun 10:672 (2019). PubMed: 30737405
  • Tian S  et al. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep 28:2517-2526.e5 (2019). PubMed: 31484065

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