Anti-EME1 antibody (ab88878)
Key features and details
- Mouse polyclonal to EME1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EME1 antibody -
Description
Mouse polyclonal to EME1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human EME1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- HeLa or Jurkat cell lysate. HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
-
Function
Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. -
Sequence similarities
Belongs to the EME1/MMS4 family. -
Cellular localization
Nucleus > nucleolus. Recruited to regions of DNA damage in S-phase cells. - Information by UniProt
-
Database links
- Entrez Gene: 146956 Human
- Omim: 610885 Human
- SwissProt: Q96AY2 Human
- Unigene: 514330 Human
-
Alternative names
- 6820428D13 antibody
- Crossover junction endonuclease EME1 antibody
- EC 3.1.22.- antibody
see all
Images
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab88878 has been referenced in 2 publications.
- Zhang H et al. TDP1-independent pathways in the process and repair of TOP1-induced DNA damage. Nat Commun 13:4240 (2022). PubMed: 35869071
- Guo Z et al. Essential meiotic structure-specific endonuclease1 (EME1) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway. Bioengineered 12:9869-9884 (2021). PubMed: 34719326