Western blot abreview for Anti-EME1 antibody

Poor
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Diluent: PBS-T
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
16 hour(s), 0 minute(s) and 0 second(s)

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Submitted Nov 12 2013

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