Immunoprecipitation abreview for Anti-EME1 antibody

Poor
Abreviews
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
1000 µg

Other product details

Concentration
1 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tris cell lysis buffer

Western Blot antibody

Name
Non-Abcam antibody was used: Human EME1 384-end
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Additional data

Additional Notes
No band detected for EME following IP with ab88878 and western blotting with in-house antibody raised against EME1 384-end.
Abcam response
Thank you for sharing this data with us. ab88878 has not previously been tested in IP and based on this abreview, it looks like this antibody does not work in this application, under these conditions. Abcam would welcome feedback from other customers.

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Verified customer

Submitted Jul 11 2014

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