Anti-Emerin antibody (ab153718)
Key features and details
- Rabbit polyclonal to Emerin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Emerin antibody
See all Emerin primary antibodies -
Description
Rabbit polyclonal to Emerin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 48-254 of Human Emerin (Uniprot ID: P50402).
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Positive control
- HeLa nuclear lysate; HeLa cells; Hela xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C. -
Tissue specificity
Skeletal muscle, heart, colon, testis, ovary and pancreas. -
Involvement in disease
Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. -
Sequence similarities
Contains 1 LEM domain. -
Post-translational
modificationsFound in four different phosphorylated forms, three of which appear to be associated with the cell cycle. -
Cellular localization
Nucleus inner membrane. Nucleus outer membrane. Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2010 Human
- Omim: 300384 Human
- SwissProt: P50402 Human
- Unigene: 522823 Human
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Alternative names
- EDMD antibody
- Emd antibody
- EMD_HUMAN antibody
see all
Images
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Anti-Emerin antibody (ab153718) at 1/5000 dilution + HeLa nuclear lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human HeLa xenograft tissue, labeling Emerin with ab153718 at 1/1000 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling Emerin with ab153718 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab153718 has been referenced in 2 publications.
- Shamloul A et al. The Methyltransferase Smyd1 Mediates LPS-Triggered Up-Regulation of IL-6 in Endothelial Cells. Cells 10:N/A (2021). PubMed: 34944023
- Bonnin E et al. Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence. PLoS Genet 14:e1007845 (2018). PubMed: 30543681