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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Emerin.
(Peptide available as ab40723)
Our Abpromise guarantee covers the use of ab40688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25, 29 kDa).|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Emerin knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab40688 observed at 35 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab40688 was shown to recognize Emerin in wild-type HAP1 cells as signal was lost at the expected MW in Emerin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Emerin knockout samples were subjected to SDS-PAGE. Ab40688 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"