Overview

  • Product name

    Anti-Emerin antibody [EMD/2167] - BSA and Azide free
    See all Emerin primary antibodies
  • Description

    Mouse monoclonal [EMD/2167] to Emerin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Emerin aa 56-167. The exact sequence is proprietary.
    Database link: P50402

  • Positive control

    • IHC-P: Human renal cell carcinoma, basal cell carcinoma and breast carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    EMD/2167
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml.

Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.

Target

  • Function

    Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
  • Tissue specificity

    Skeletal muscle, heart, colon, testis, ovary and pancreas.
  • Involvement in disease

    Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.
  • Sequence similarities

    Contains 1 LEM domain.
  • Post-translational
    modifications

    Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.
  • Cellular localization

    Nucleus inner membrane. Nucleus outer membrane. Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EDMD antibody
    • Emd antibody
    • EMD_HUMAN antibody
    • Emerin antibody
    • Emery Dreifuss muscular dystrophy antibody
    • STA antibody
    see all

Images

  • Anti-Emerin antibody [EMD/2167] - BSA and Azide free (ab237832) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 29 kDa

  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for Emerin with ab237832 at 2 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human basal cell carcinoma tissue stained for Emerin with ab237832 at 2 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Emerin with ab237832 at 2 µg/ml in immunohistochemical analysis.

References

ab237832 has not yet been referenced specifically in any publications.

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