Overview

  • Product name

    Anti-Emi1 antibody [EMI1/1176]
    See all Emi1 primary antibodies
  • Description

    Mouse monoclonal [EMI1/1176] to Emi1
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Emi1 aa 1-250. The exact sequence is proprietary. corresponding to a fragment of 203 amino acids.
    Database link: Q9UKT4

  • Positive control

    • HeLa, 293, K562 or HepG2 cell lysates. Ovarian carcinoma tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    EMI1/1176
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • Function

    Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 1 IBR-type zinc finger.
  • Developmental stage

    Accumulates in late G1 phase, levels rise during S phase and drop in early mitosis.
  • Domain

    The C-terminal region is required for inhibition of APC activity.
  • Post-translational
    modifications

    Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Early mitotic inhibitor 1 antibody
    • Emi 1 antibody
    • EMI1 antibody
    • F box only protein 5 antibody
    • F box protein 5 antibody
    • F box protein Fbx 5 antibody
    • F box protein Fbx5 antibody
    • F-box only protein 5 antibody
    • FBX5 antibody
    • FBX5_HUMAN antibody
    • Fbxo31 antibody
    • fbxo5 antibody
    see all

Images

  • ab215765 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • All lanes : Anti-Emi1 antibody [EMI1/1176] (ab215765) at 2 µg/ml

    Lane 1 : HeLa cell lysates
    Lane 2 : HepG2 cell lysates
    Lane 3 : HEK293 cell lysates
    Lane 4 : K562 cell lysates

    Predicted band size: 50 kDa

  • Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.

  • Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.

References

ab215765 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Formaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 09 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up