Overview

  • Product name
    Anti-Emi1 antibody [EMI1/1176] - BSA and Azide free
    See all Emi1 primary antibodies
  • Description
    Mouse monoclonal [EMI1/1176] to Emi1 - BSA and Azide free
  • Host species
    Mouse
  • Tested applications
    Suitable for: Protein Array, Flow Cyt, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Emi1 aa 1-250. The exact sequence is proprietary. recombinant fragment of 203 amino acid residues
    Database link: Q9UKT4

  • Positive control
    • HeLa, HEK293, K562 or HepG2 cells. Human Ovarian carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Clonality
    Monoclonal
  • Clone number
    EMI1/1176
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
Flow Cyt Use 0.5-1µg for 106 cells.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 1 IBR-type zinc finger.
  • Developmental stage
    Accumulates in late G1 phase, levels rise during S phase and drop in early mitosis.
  • Domain
    The C-terminal region is required for inhibition of APC activity.
  • Post-translational
    modifications
    Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early mitotic inhibitor 1 antibody
    • Emi 1 antibody
    • EMI1 antibody
    • F box only protein 5 antibody
    • F box protein 5 antibody
    • F box protein Fbx 5 antibody
    • F box protein Fbx5 antibody
    • F-box only protein 5 antibody
    • FBX5 antibody
    • FBX5_HUMAN antibody
    • Fbxo31 antibody
    • fbxo5 antibody
    see all

Images

  • This data was produced with ab215765, the same antibody in a different formulation with BSA and Azide.
    ab215765 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Immunohistochemical analysis of paraffin embedded, formalin fixed human ovarian carcinoma tissue labeling Emi1 with ab212397 at 1 μg/ml

  • Immunohistochemical analysis of paraffin embedded, formalin fixed human ovarian carcinoma tissue labeling Emi1 with ab212397 at 1 μg/ml.

  • All lanes : Anti-Emi1 antibody [EMI1/1176] - BSA and Azide free (ab212397) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HEK293 cell lysate
    Lane 4 : K562 cell lysate

    Predicted band size: 50 kDa

References

ab212397 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 25 2017

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