Overview

  • Product name

    Anti-Emi1 antibody [EPR15320-103]
    See all Emi1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15320-103] to Emi1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Emi1 aa 100-250. The exact sequence is proprietary.
    Database link: Q9UKT4

  • Positive control

    • HeLa, 293, HepG2 whole cell lysate (ab7900); Human ovarian carcinoma and mouse liver tissues; HeLa and HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15320-103
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/30 - 1/40.

Target

  • Function

    Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 1 IBR-type zinc finger.
  • Developmental stage

    Accumulates in late G1 phase, levels rise during S phase and drop in early mitosis.
  • Domain

    The C-terminal region is required for inhibition of APC activity.
  • Post-translational
    modifications

    Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Early mitotic inhibitor 1 antibody
    • Emi 1 antibody
    • EMI1 antibody
    • F box only protein 5 antibody
    • F box protein 5 antibody
    • F box protein Fbx 5 antibody
    • F box protein Fbx5 antibody
    • F-box only protein 5 antibody
    • FBX5 antibody
    • FBX5_HUMAN antibody
    • Fbxo31 antibody
    • fbxo5 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Emi1 with ab187144 at 1/100 with a  prediluted HRP Polymer for Rabbit IgG as secondary antibody.

  • All lanes : Anti-Emi1 antibody [EPR15320-103] (ab187144) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation of Emi1 from HepG2 cell lysate using ab187144 at 1/50 (lane 1) or 1X PBS (negative control) (lane 2). Immunostained with ab187144 at 1/1000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunofluorescent analysis of HepG2 cells labeling Emi1 with ab187144 at 1/250 and DAPI staining (blue).

  • Immunofluorescent analysis of HeLa cells labeling Emi1 with ab187144 at 1/100 and DAPI staining (blue).

  • Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling Emi1 with ab187144 at 1/100 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.

References

This product has been referenced in:

  • Liu L  et al. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. Biomed Res Int 2018:7849294 (2018). Read more (PubMed: 29850565) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 14 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 09 2017

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