Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15320] to Emi1
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-Emi1 antibody [EPR15320]
See all Emi1 primary antibodies
DescriptionRabbit monoclonal [EPR15320] to Emi1
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Emi1 aa 100-250. The exact sequence is proprietary.
Database link: Q9UKT4
- HepG2 and HeLa cells and cell lysates; 293 and K562 cell lysates; Human ovarian carcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionRegulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.
Sequence similaritiesContains 1 F-box domain.
Contains 1 IBR-type zinc finger.
Developmental stageAccumulates in late G1 phase, levels rise during S phase and drop in early mitosis.
DomainThe C-terminal region is required for inhibition of APC activity.
modificationsPhosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.
- Information by UniProt
- Early mitotic inhibitor 1 antibody
- Emi 1 antibody
- EMI1 antibody
Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody. The slide on the right is counter stained with Dapi (blue).
All lanes : Anti-Emi1 antibody [EPR15320] (ab184950) at 1/5000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Emi1 with ab184950 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/180 dilution (red). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (green).
ab184950 has not yet been referenced specifically in any publications.