Anti-EMILIN1 antibody (ab231052)
Key features and details
- Rabbit polyclonal to EMILIN1
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-EMILIN1 antibody
See all EMILIN1 primary antibodies -
Description
Rabbit polyclonal to EMILIN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Rat EMILIN1 aa 650 to the C-terminus. (Expressed in E.coli).
Database link: D3Z9E1 -
Positive control
- IHC-P: Rat spleen tissue. WB: Rat serum; Rat intestine lysate; Recombinant rat EMILIN1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231052 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 94 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 94 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity. -
Tissue specificity
Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain.
Contains 1 EMI domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils. - Information by UniProt
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Database links
- Entrez Gene: 11117 Human
- Entrez Gene: 100952 Mouse
- Entrez Gene: 298845 Rat
- Omim: 130660 Human
- SwissProt: Q9Y6C2 Human
- SwissProt: Q99K41 Mouse
- Unigene: 63348 Human
- Unigene: 286375 Mouse
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Alternative names
- Elastin microfibril interface located protein 1 antibody
- Elastin microfibril interface located protein antibody
- Elastin microfibril interface-located protein 1 antibody
see all
Images
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Paraffin-embedded rat spleen tissue stained for EMILIN1 using ab231052 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
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Paraffin-embedded rat spleen tissue stained for EMILIN1 using ab231052 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
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All lanes : Anti-EMILIN1 antibody (ab231052) at 3 µg/ml
Lane 1 : Rat serum
Lane 2 : Rat intestine lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 94 kDa -
Anti-EMILIN1 antibody (ab231052) at 3 µg/ml + Recombinant rat EMILIN1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 94 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231052 has not yet been referenced specifically in any publications.