Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14678] to EMILIN1 - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-EMILIN1 antibody [EPR14678] - N-terminal
See all EMILIN1 primary antibodies
DescriptionRabbit monoclonal [EPR14678] to EMILIN1 - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human EMILIN1 aa 100-300 (N terminal). The exact sequence is proprietary.
Database link: Q9Y6C2
- Human uterus, fetal kidney and colon cancer tissue lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/30 - 1/50.|
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 107 kDa).|
FunctionMay be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
Tissue specificityDistributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression.
Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
Contains 1 EMI domain.
Cellular localizationSecreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils.
- Information by UniProt
- Elastin microfibril interface located protein 1 antibody
- Elastin microfibril interface located protein antibody
- Elastin microfibril interface-located protein 1 antibody
All lanes : Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953) at 1/50000 dilution
Lane 1 : Human uterus tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human colon cancer tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 107 kDa
Western blot analysis of immunoprecipitation pellet from Human fetal kidney tissue lysate labeling EMILIN1 with ab185953 at a 1/50 dilution. Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.
ab185953 has been referenced in 1 publication.
- Rabajdová M et al. Detection of Pathological Changes in the Aorta during Thoracic Aortic Aneurysm Progression on Molecular Level. Dis Markers 2017:9185934 (2017). PubMed: 29158612