Recombinant
RabMAb

Recombinant Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)

Overview

  • Product name

    Anti-EMILIN1 antibody [EPR14678] - N-terminal
    See all EMILIN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14678] to EMILIN1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EMILIN1 aa 100-300 (N terminal). The exact sequence is proprietary.
    Database link: Q9Y6C2

  • Positive control

    • Human uterus, fetal kidney and colon cancer tissue lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30 - 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 107 kDa).

Target

  • Function

    May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
  • Tissue specificity

    Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
    Contains 1 EMI domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils.
  • Information by UniProt
  • Database links

  • Alternative names

    • Elastin microfibril interface located protein 1 antibody
    • Elastin microfibril interface located protein antibody
    • Elastin microfibril interface-located protein 1 antibody
    • Elastin microfibril interfacer 1 antibody
    • EMIL1_HUMAN antibody
    • EMILIN 1 antibody
    • EMILIN antibody
    • EMILIN-1 antibody
    • Emilin1 antibody
    • gp115 antibody
    see all

Images

  • All lanes : Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953) at 1/50000 dilution

    Lane 1 : Human uterus tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : Human colon cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 107 kDa

  • Western blot analysis of immunoprecipitation pellet from Human fetal kidney tissue lysate labeling EMILIN1 with ab185953 at a 1/50 dilution. Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

References

This product has been referenced in:

  • Rabajdová M  et al. Detection of Pathological Changes in the Aorta during Thoracic Aortic Aneurysm Progression on Molecular Level. Dis Markers 2017:9185934 (2017). Read more (PubMed: 29158612) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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