Anti-EML1 antibody (ab204174)
Key features and details
- Rabbit polyclonal to EML1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EML1 antibody
See all EML1 primary antibodies -
Description
Rabbit polyclonal to EML1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EML1 aa 100-200.
Database link: O00423 -
Positive control
- A549 cells; Human cerebral cortex tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
-
Function
May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. -
Tissue specificity
Ubiquitous; expressed in most tissues with the exception of thymus and peripheral blood lymphocytes. -
Sequence similarities
Belongs to the WD repeat EMAP family.
Contains 10 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 2009 Human
- GenBank: BC032541 Human
- Omim: 602033 Human
- SwissProt: O00423 Human
- Unigene: 12451 Human
-
Alternative names
- echinoderm microtubule associated protein like 1 antibody
- Echinoderm microtubule-associated protein-like 1 antibody
- ELP79 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab204174 has not yet been referenced specifically in any publications.