Overview

  • Product name

  • Description

    Rabbit polyclonal to EML1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human EML1 aa 115-164.
    Sequence:

    SGVRKETAVPATKSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGSTSSS


    Database link: O00423

  • Positive control

    • A549 cells; Human cerebral cortex tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
  • Tissue specificity

    Ubiquitous; expressed in most tissues with the exception of thymus and peripheral blood lymphocytes.
  • Sequence similarities

    Belongs to the WD repeat EMAP family.
    Contains 10 WD repeats.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • echinoderm microtubule associated protein like 1 antibody
    • Echinoderm microtubule-associated protein-like 1 antibody
    • ELP79 antibody
    • EMAL1_HUMAN antibody
    • EMAP antibody
    • EMAP-1 antibody
    • EMAPL antibody
    • Eml1 antibody
    • FLJ45033 antibody
    • HuEMAP antibody
    • HuEMAP-1 antibody
    see all

Images

  • Immunofluorescent analysis of PFA-fixed,Triton X-100 permeabilized A549 cells labeling EML1 with ab204174 at 4 µg/ml (green).

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling EML1 with ab204174 at 1/200 dilution.

References

ab204174 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204174.
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