Anti-EML1 antibody - C-terminal (ab153703)

Overview

  • Product name
    Anti-EML1 antibody - C-terminal
    See all EML1 primary antibodies
  • Description
    Rabbit polyclonal to EML1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 585-815 of Human EML1 (UniProt: O00423).

  • Positive control
    • HepG2 whole cell lysate; A549 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7,00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Glycine, 0.75% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-EML1 antibody - C-terminal (ab153703) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Performed under reducing conditions.


    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A549 cells labeling EML1 with ab153703 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab153703 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
NSC34
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 19 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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