Overview

  • Product name

    Anti-EML2 antibody
  • Description

    Rabbit polyclonal to EML2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 201-438 of Human EML2 (O95834).

  • Positive control

    • HepG2 and HCT116 cell lysates. HeLa cells. Human H441 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-EML2 antibody (ab153979) at 1/500 dilution + HCT116 whole cell lysate
    at 30 µg

    Predicted band size: 71 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling EML2 with ab153979 at 1/200 dilution. Lower panel is costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human H441 xenograft tissue labeling EML2 with ab153979 at 1/100 dilution.

References

ab153979 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153979.
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