Key features and details
- Rabbit polyclonal to EML4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EML4 antibody
See all EML4 primary antibodies
DescriptionRabbit polyclonal to EML4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla
Synthetic peptide, corresponding to a region within amino acids 931-981 of Human EML4 (NP_061936.2)
- HeLa whole cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 109 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
Sequence similaritiesBelongs to the WD repeat EMAP family.
Contains 9 WD repeats.
Developmental stageStrongly overexpressed during mitosis.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- EML4 antibody
- C2orf2 antibody
- Echinoderm microtubule associated protein like 4 antibody
All lanes : Anti-EML4 antibody (ab85834) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 60 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EML4 with ab85834 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of ab85834
Lane 1: ab85834 at 3µg/mg whole cell lysate.Lane 2: Control IgG.
ab85834 at 0.4µg/ml for WB.Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
ab85834 has not yet been referenced specifically in any publications.