Key features and details
- Rabbit polyclonal to EML4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EML4 antibody
See all EML4 primary antibodies
DescriptionRabbit polyclonal to EML4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 150 and 200 of human EML4 (NP_061936.2)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86062 was affinity purified using an epitope specific to EML4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab86062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 109 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
Sequence similaritiesBelongs to the WD repeat EMAP family.
Contains 9 WD repeats.
Developmental stageStrongly overexpressed during mitosis.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- EML4 antibody
- C2orf2 antibody
- Echinoderm microtubule associated protein like 4 antibody
All lanes : Anti-EML4 antibody (ab86062) at 1/0.04 dilution
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 109 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lin plastica stomach cancer tissue labelling EML4 with ab86062 at 1/200 (1µg/ml). Detection: DAB.
Detection of EML4 by Western Blot of Immunprecipitate.
ab86062 at 0.4µg/ml staining EML4 in HeLa whole cell lysate immunoprecipitated using ab86062 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab86062 has not yet been referenced specifically in any publications.