Anti-EMP-1 antibody - N-terminal (ab230445)
Key features and details
- Rabbit polyclonal to EMP-1 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EMP-1 antibody - N-terminal
See all EMP-1 primary antibodies -
Description
Rabbit polyclonal to EMP-1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human EMP-1 aa 35-63 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P54849 -
Positive control
- IHC-P: Human kidney and adrenal tissues. WB: K562 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/75.
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WB |
1/1000. Predicted molecular weight: 18 kDa.
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Notes |
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IHC-P
1/75. |
WB
1/1000. Predicted molecular weight: 18 kDa. |
Target
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Sequence similarities
Belongs to the PMP-22/EMP/MP20 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2012 Human
- Omim: 602333 Human
- SwissProt: P54849 Human
- Unigene: 719042 Human
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Alternative names
- CL-20 antibody
- EMP-1 antibody
- EMP1 antibody
see all
Images
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Anti-EMP-1 antibody - N-terminal (ab230445) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg
Predicted band size: 18 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for EMP-1 using ab230445 at 1/75 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human adrenal tissue stained for EMP-1 using ab230445 at 1/75 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab230445 has been referenced in 3 publications.
- Liu Y et al. EMP1 Promotes the Proliferation and Invasion of Ovarian Cancer Cells Through Activating the MAPK Pathway. Onco Targets Ther 13:2047-2055 (2020). PubMed: 32210572
- Lin B et al. High expression of EMP1 predicts a poor prognosis and correlates with immune infiltrates in bladder urothelial carcinoma. Oncol Lett 20:2840-2854 (2020). PubMed: 32782602
- Busch M et al. p53, miR-34a and EMP1-Newly Identified Targets of TFF3 Signaling in Y79 Retinoblastoma Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31450568