Anti-EMP2 antibody (ab223718)
Key features and details
- Rabbit polyclonal to EMP2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EMP2 antibody
See all EMP2 primary antibodies -
Description
Rabbit polyclonal to EMP2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment corresponding to Human EMP2 aa 1-100.
Database link: P54851 -
Positive control
- IHC-P: Human kidney, skin and lung tissue. WB: MCF7 cell lysate. ICC/IF: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 19 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 19 kDa. |
Target
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Sequence similarities
Belongs to the PMP-22/EMP/MP20 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 453910 Chimpanzee
- Entrez Gene: 2013 Human
- Omim: 602334 Human
- SwissProt: A5A6N6 Chimpanzee
- SwissProt: P54851 Human
- Unigene: 531561 Human
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Alternative names
- EMP 2 antibody
- EMP-2 antibody
- EMP2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP2 antibody (ab223718)
Paraffin-embedded human kidney tissue stained for EMP2 using ab223718 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP2 antibody (ab223718)Paraffin-embedded human skin tissue stained for EMP2 using ab223718 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP2 antibody (ab223718)Paraffin-embedded human lung tissue stained for EMP2 using ab223718 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-EMP2 antibody (ab223718)Anti-EMP2 antibody (ab223718) at 1/250 dilution + MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 19 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-EMP2 antibody (ab223718)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for EMP2 (green) using ab223718 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab223718 has not yet been referenced specifically in any publications.
