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  1. Link

    emp2-antibody-ab223718.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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Anti-EMP2 antibody (ab223718)

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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP2 antibody (ab223718)
  • Western blot - Anti-EMP2 antibody (ab223718)
  • Immunocytochemistry/ Immunofluorescence - Anti-EMP2 antibody (ab223718)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-EMP2 antibody
    See all EMP2 primary antibodies
  • Description

    Rabbit polyclonal to EMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment corresponding to Human EMP2 aa 25-60.
    Sequence:

    DNAWWVGDEFFADVWRICTNNTNCTVINDSFQEYST


    Database link: P54851

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human lung tissue. WB: MCF7 cell lysate. ICC/IF: U-251 MG cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • FAK / PYK
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • FAK family
    • Cancer
    • Tumor biomarkers
    • Other
    • Developmental Biology
    • Reproduction
    • Placental development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MCF7 whole cell lysate (ab29537)
    • MCF7 whole cell lysate (ab3871)
  • Recombinant Protein

    • Recombinant Human EMP2 protein (ab152364)
  • Related Products

    • Recombinant Human EMP2 protein (ab152364)

Applications

Our Abpromise guarantee covers the use of ab223718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 19 kDa.

Target

  • Sequence similarities

    Belongs to the PMP-22/EMP/MP20 family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P54851 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 453910 Chimpanzee
    • Entrez Gene: 2013 Human
    • Omim: 602334 Human
    • SwissProt: A5A6N6 Chimpanzee
    • SwissProt: P54851 Human
    • Unigene: 531561 Human
    • Alternative names

      • EMP 2 antibody
      • EMP-2 antibody
      • EMP2 antibody
      • EMP2_HUMAN antibody
      • Epithelial membrane protein 2 antibody
      • Protein XMP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP2 antibody (ab223718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP2 antibody (ab223718)

      Paraffin-embedded human lung tissue stained for EMP2 using ab223718 at 1/50 dilution in immunohistochemical analysis.

    • Western blot - Anti-EMP2 antibody (ab223718)
      Western blot - Anti-EMP2 antibody (ab223718)
      Anti-EMP2 antibody (ab223718) at 1/250 dilution + MCF7 (human breast adenocarcinoma cell line) cell lysate

      Predicted band size: 19 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-EMP2 antibody (ab223718)
      Immunocytochemistry/ Immunofluorescence - Anti-EMP2 antibody (ab223718)

      PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for EMP2 (green) using ab223718 at 4 µg/ml in ICC/IF.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    ab223718 has not yet been referenced specifically in any publications.

    Publishing research using ab223718? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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