Key features and details
- Rabbit polyclonal to EMP2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EMP2 antibody
See all EMP2 primary antibodies
DescriptionRabbit polyclonal to EMP2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- IHC-P: Human lung tissue. WB: MCF7 cell lysate. ICC/IF: U-251 MG cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab223718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 19 kDa.|
Sequence similaritiesBelongs to the PMP-22/EMP/MP20 family.
- Information by UniProt
- EMP 2 antibody
- EMP-2 antibody
- EMP2 antibody
Paraffin-embedded human lung tissue stained for EMP2 using ab223718 at 1/50 dilution in immunohistochemical analysis.
Anti-EMP2 antibody (ab223718) at 1/250 dilution + MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 19 kDa
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for EMP2 (green) using ab223718 at 4 µg/ml in ICC/IF.
ab223718 has not yet been referenced specifically in any publications.