RecombinantRabMAb

Recombinant Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)

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Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
  • Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
  • Flow Cytometry - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
  • Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
  • Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23225-94] to EMR1/ADGRE1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-EMR1/ADGRE1 antibody [EPR23225-94]
    See all EMR1/ADGRE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23225-94] to EMR1/ADGRE1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human EMR1/ADGRE1 aa 400-550. The exact sequence is proprietary.
    Database link: Q14246

  • Positive control

    • WB: Human spleen, colon and stomach lysates. IHC-P: Human gastric cancer and colon tissues. ICC/IF: HEK-293 cells transfected with myc-tagged EMR1/ADGRE1 expression vector. Flow Cyt: HEK-293 cells transfected with myc-tagged EMR1/ADGRE1 expression vector.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23225-94

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab254293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).
IHC-P 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

    • Tissue specificity

      Expression is restricted to eosinophils.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
      Contains 6 EGF-like domains.
      Contains 1 GPS domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Adgre1 antibody
        • Adhesion G protein-coupled receptor E1 antibody
        • AGRE1_HUMAN antibody
        • EGF-like module receptor 1 antibody
        • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
        • EMR1 hormone receptor antibody
        see all

      Images

      • Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        All lanes : Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293) at 1/1000 dilution

        Lane 1 : Human colon tissue lysate
        Lane 2 : Human stomach tissue lysate

        Lysates/proteins at 40 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 97 kDa
        Observed band size: 100 kDa
        why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        Exposure time: 70 seconds.

      • Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        All lanes : Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293) at 1/1000 dilution

        Lane 1 : Human spleen tissue lysate
        Lane 2 : Human brain tissue lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 97 kDa
        Observed band size: 100 kDa why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        This blot was developed using a higher sensitivity ECL substrate.

        Negative control: Human brain (PMID:17823986). 

        Exposure time: 3 minutes.

      • Flow Cytometry - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        Flow Cytometry - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)

        Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HEK-293T (transfected with myc tagged EMR1/ADGRE1 construct) cells labelling EMR1/ADGRE1 with ab254293 at 1/500 (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

        Cells were surface stained with isotype control (ab172730) or ab254293. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

      • Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 cells labelling EMR1/ADGRE1 with ab254293 at 1/100 dilution, followed by antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293 cell line transfected with myc-tagged EMR1/ADGRE1 expression vector. 2233S Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is at 1000 dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EMR1/ADGRE1 with ab254293 at 1/16000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in eosinophils of human colon (PMID:17823986).The section was incubated with ab254293 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)

        Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling EMR1/ADGRE1 with ab254293 at 1/16000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in eosinophils of human gastric cancer (PMID:17823986).The section was incubated with ab254293 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
        Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab254293? Please let us know so that we can cite the reference in this datasheet.

      ab254293 has not yet been referenced specifically in any publications.

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