Overview

  • Product name

  • Description

    Rabbit polyclonal to EMRE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human EMRE aa 7-52.
    Sequence:

    RWLVLAPVRSGALRSGPSLRKDGDVSAAWSGSGRSLVPSRSVIVTR

  • Positive control

    • Human kidney tissue; HEK293T cell EMRE over-expression lysate.
  • General notes

     This product was previously labelled as C22orf32

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • All lanes : Anti-EMRE antibody (ab122209) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)

    Lane 2 : EMRE over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli and nucleus but not nucleoli. Recommended concentration of ab122209 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122209 at 1/150 dilution staining EMRE in paraffin-embedded Human Kidney tissue by Immunohistochemistry.

References

ab122209 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse heart)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
5 µg
Specification
Mouse heart
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 4% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 18 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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