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    emsy-antibody-ab123.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Methyl Lysine
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Anti-EMSY antibody (ab123)

  • Datasheet
Submit a review Q&A (3)References (5)

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Immunoprecipitation - Anti-EMSY antibody (ab123)
  • Immunoprecipitation - Anti-EMSY antibody (ab123)
  • Western blot - Anti-EMSY antibody (ab123)

Key features and details

  • Rabbit polyclonal to EMSY
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EMSY antibody
    See all EMSY primary antibodies
  • Description

    Rabbit polyclonal to EMSY
  • Host species

    Rabbit
  • Specificity

    This antibody detects a band of the same size by Western blot as ab4579, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years after this antibody). The band detected by this antibody also runs at the same height as full length in vitro translated EMSY. This confirms that the band detected is EMSY.
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human EMSY aa 100-200.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methyl Lysine
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
1/3000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa). Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD. However, the independently raised ab4579 has been used to confirm the specificity of this antibody.
IP
1/3000.
Notes
WB
1/3000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa). Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD. However, the independently raised ab4579 has been used to confirm the specificity of this antibody.
IP
1/3000.

Target

  • Target information above from: UniProt accession Q7Z589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 56946 Human
    • Entrez Gene: 233545 Mouse
    • Omim: 608574 Human
    • SwissProt: Q7Z589 Human
    • SwissProt: Q8BMB0 Mouse
    • Unigene: 352588 Human
    • Unigene: 387819 Mouse
    • Alternative names

      • C11orf30 antibody
      • Chromosome 11 open reading frame 30 antibody
      • Emsy antibody
      • EMSY protein antibody
      • EMSY_HUMAN antibody
      • FLJ90741 antibody
      • GL002 antibody
      • Protein EMSY antibody
      see all

    Images

    • Immunoprecipitation - Anti-EMSY antibody (ab123)
      Immunoprecipitation - Anti-EMSY antibody (ab123)Hughes-Davies et al, 2003

      WB using EMSY antibody (ab123) demonstrating the interaction between EMSY and BRCA2 by immunoprecipitation.

      Endogenous coimmunoprecipitation of EMSY and BRCA2 from unmanipulated asynchronously dividing HeLa cells.

      Immunoprecipitation with an unrelated antibody (anti-GFP), preimmune serum, ab123 or anti-BRCA2 antibody was followed by Western blotting with ab123.

      Neither of the unrelated antibodies could coprecipitate EMSY, whereas an EMSY signal was detected in the immunoprecipitate obtained with the anti-BRCA2 antibody. The anti-EMSY antibody could also immunoprecipitate endogenous EMSY protein.

    • Immunoprecipitation - Anti-EMSY antibody (ab123)
      Immunoprecipitation - Anti-EMSY antibody (ab123)

      EMSY was immunoprecipitated using 0.5mg MCF7 whole cell extract, Rabbit polyclonal to EMSY diluted to 1/3,000 in RIPA buffer and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab123.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 141kDa; EMSY

    • Western blot - Anti-EMSY antibody (ab123)
      Western blot - Anti-EMSY antibody (ab123)

      Western blot of EMSY on MCF-7 cell lysate.

      Lane 1: ab4579 at 1/500
      Lane 2: ab123 at 1/500.

      Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.

      Secondary ab Lane 1: Rabbit polyclonal to Goat IgG H&L (HRP) ab6741 (1/5000)
      Exposure time: 3 min.

      Secondary ab Lane 2: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
      Exposure time: 10 sec.

      Lysate at 20µg/lane.

      Lane 1 - Exposure time : 3 min.
      Lane 2 - Exposure time : 10 sec.

      Variability in the size at which EMSY runs by Western blot has been exper

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (5)

    Publishing research using ab123? Please let us know so that we can cite the reference in this datasheet.

    ab123 has been referenced in 5 publications.

    • Kolinjivadi AM  et al. Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments. Mol Cell 67:867-881.e7 (2017). PubMed: 28757209
    • Altinisik J  et al. Expression of EMSY gene in sporadic ovarian cancer. Mol Biol Rep 38:359-63 (2011). WB ; Human . PubMed: 20349280
    • Wang Z  et al. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal 3:ra2 (2010). PubMed: 20068230
    • Seedhouse CH  et al. DNA repair contributes to the drug-resistant phenotype of primary acute myeloid leukaemia cells with FLT3 internal tandem duplications and is reversed by the FLT3 inhibitor PKC412. Leukemia 20:2130-6 (2006). PubMed: 17066094
    • Hughes-Davies L  et al. EMSY Links the BRCA2 Pathway to Sporadic Breast and Ovarian Cancer. Cell 115:523-35 (2003). ICC/IF, IP ; Mouse . PubMed: 14651845

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    May I use your antibody ab123 together with blocking peptide from Bethyl: http://www.bethyl.com/product/BP300-253 ?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2012

    Answer

    Thank you for contacting us.
    Following on from my email dated 21st November, I have looked into ab123 and whether we have the blocking peptide. Unfortunately on this occasion we worked with a collaborator lab to produce the antibody and therefore we don't have the blocking peptide to add to our catalogue.
    I am very sorry that on this occasion I was not able to provide you with a more positive outcome. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 30 2012

    Question

    May I use your antibody ab123 together with blocking peptide from Bethyl: http://www.bethyl.com/product/BP300-253 ?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2012

    Answer

    Thank you for contacting us.
    This blocking peptide from bethyl is for use with their product A300-253A . Since we do not know the sequence of this peptide, we cannot tell you for certain whether it is suitable for use with ab123.
    Currently, we do not supply the blocking peptide for ab123 but I have contacted the lab to see whether it is possible to add it to the catalogue.
    I will let you know when I get their response.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 21 2012

    Question

    Customer is interested in using an EMSY antibody for Western blotting. On the WB image for ab4580 there is a fairly strong band at 37 kDa seen. For ab123, the band at 37 kDa is present but is fainter. Is this band always seen with these two antibodies?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 09 2005

    Answer

    Thank you for your enquiry. I consulted with a colleague and this band at approximately 37 kDa that is not always seen with these two antibodies. We are not sure what is it is, but it may possibly be a degradation product. If you have any additional questions, please do contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jun 14 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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