EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)

Overview

  • Product name

    EMT Marker / Epithelial to Mesenchymal Transition Marker Panel
  • Product overview

    EMT Marker / Epithelial to Mesenchymal Marker antibody sampler panel ab216833 contains high performing antibodies against the key EMT markers: E-cadherin,  N-cadherin, Snail + Slug, and Vimentin. It also contains an anti-rabbit secondary antibody.


    EMT is a reversible process where epithelial cells undergo defined molecular changes to become motile mesenchymal cells. This EMT marker sampler panel is a cost-effective and convenient means of evaluating EMT.


    The antibodies in this panel were selected for their exceptional performance in IHC in human tissues. These antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information.


    We do not recommend the use of SNAIL + SLUG antibody ab85936 for western blot (note: the other antibodies in this panel perform well in western blot). For SNAIL + SLUG in western blot, we recommend ab180714.


    Panel contents:


    E-cadherin rabbit monoclonal ab40772 - 10 µl
    N-cadherin rabbit monoclonal ab76011 - 10 µl
    Snail + Slug rabbit polyclonal ab85936 - 10 µg
    Vimentin rabbit monoclonal ab92547 - 10 µl
    anti-rabbit secondary antibody (HRP) ab205718 - 100 µg

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.

Properties

Images

  • Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab40772. Green-E-Cadherin red-PI

  • IHC image of ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85936, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC image of unpurified ab92547 staining Vimentin in human breast adenocarcinoma formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92547, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab216833 has not yet been referenced specifically in any publications.

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