Anti-Emx1 antibody (ab32925)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.

Overview

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
IHC-P
  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide (ab32995).

    IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

  • Target

    • Function
      Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
    • Tissue specificity
      Cerebral cortex.
    • Sequence similarities
      Belongs to the EMX homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Empty spiracles homeobox 1 antibody
      • Empty spiracles homolog 1 antibody
      • empty spiracles homolog 1 Drosophila antibody
      • Empty spiracles like protein 1 antibody
      • Empty spiracles-like protein 1 antibody
      • EMX1 antibody
      • EMX1_HUMAN antibody
      • Homeobox protein EMX1 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Emx1 expression in adult rat cerebellum, using ab32925 at 1/200 dilution.
    • Emx1 expression in rat striatum, using ab32925 at 1/200 dilution.
    • Emx1 staining of the rat primary motor cortex using ab32925 at 1/200.

    References

    ab32925 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your enquiry and for your patience. It has taken some time to discuss this with our source for this antibody.

    It is unfortunately difficult to say what the lighter brown staining is exactly in the IHC image provided for ab23340. A western blot with this tissue (human spermatophore epithelial cells) would have to be run to determine if any non-specific staining is there. As this protein (RAX) is a transcription factor, and this IHC image includes the nuclear stain, DAPI, the brown/blue staining is the specific signal here. The lighter brown is cytosolic staining, which could be non specific. It may be the experiment required further optimization in this case and I am sorry we have no further information on this occasion as it has been tested externally.

    Regarding EMX1 antibodies, we have several available. However, I am sorry that regrettably non of these have been tested in IHC-Fr (cryosections).

    ab32925 Emx1 antibody
    Rabbit polyclonal
    Reacts with: Rat
    Please note this is a fast track antibody which has not yet been fully characterised. Further information on the terms and conditions for using Fast Track antibodies can be found from the datasheet:
    https://www.abcam.com/index.html?datasheet=32925 https://www.abcam.com/index.html?datasheet=32925.

    ab82901 Emx1 antibody
    Rabbit polyclonal
    Tested in: WB
    Reacts with: Human
    https://www.abcam.com/index.html?datasheet=82901 https://www.abcam.com/index.html?datasheet=82901.

    ab125443 Anti-Emx1 antibody
    Rabbit polyclonal
    Tested in: WB
    Reacts with: Mouse
    https://www.abcam.com/index.html?datasheet=125443 https://www.abcam.com/index.html?datasheet=125443.

    If you would like to test one of the non Fast Track antibodies in this untested application, please do not hestitate to contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:
    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse E17 forebrain)
    Specification
    Mouse E17 forebrain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    Yes - 0.025% TX100
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Aug 21 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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