Overview

  • Product name

  • Description

    Rabbit polyclonal to EMX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Dog, Human, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Mouse EMX2 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab109642)

  • Positive control

    • This antibody gave a positive signal in both Mouse brain and hippocampus tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab94713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
  • Tissue specificity

    Cerebral cortex.
  • Involvement in disease

    Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid.
  • Sequence similarities

    Belongs to the EMX homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Empty spiracles homeobox 2 antibody
    • Empty spiracles homolog 2 (Drosophila) antibody
    • Empty spiracles homolog 2 antibody
    • Empty spiracles like protein 2 antibody
    • Empty spiracles-like protein 2 antibody
    • EMX 2 antibody
    • EMX2 antibody
    • EMX2_HUMAN antibody
    • Homeobox protein EMX 2 antibody
    • Homeobox protein EMX2 antibody
    see all

Images

  • All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml

    Lane 1 : Mouse Hippocampus Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Additional bands at: 260 kDa (possible non-specific binding), 76 kDa (possible non-specific binding)


    Exposure time: 16 minutes

References

ab94713 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus tropicalis Cell (Tadpole whole-mount)
Permeabilization
Yes - 0.1% TritonX100
Specification
Tadpole whole-mount
Blocking step
CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Helen Willsey

Verified customer

Submitted May 01 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
BRAIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Kyungjoo Seong

Verified customer

Submitted Nov 30 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (E14 mouse ES cells transduced with lentiviral EMX2)
Specification
E14 mouse ES cells transduced with lentiviral EMX2
Permeabilization
Yes - 10min Triton X-100 RT
Fixative
Paraformaldehyde

Mr. Sir or Madam Albrecht

Verified customer

Submitted Dec 08 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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