Human Albumin ELISA Kit

As of December 12, 2019 Human Albumin ELISA kit has been re-developed with new recombinant monoclonal antibodies to provide improved performance and consistency.

Human Albumin ELISA kit (ab179887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Albumin with 100 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes native human Albumin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, cell and tissue extract samples have not been tested with this kit.

SPECIES REACTIVITY

This kit recognizes human Albumin and Cynomolgus monkey albumin protein. If you require a kit that is human-specific, please see ab227933.

Other species reactivity was determined by measuring 1:4,000,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.

Reactivity < 3% was determined for the following species: Mouse, rat, Cow.

Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.