Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha 1 Antitrypsin (SERPINA1) protein in cit plasma, hep plasma, serum, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human alpha 1 Antitrypsin (SERPINA1) with 13 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human alpha 1 Antitrypsin protein in serum and plasma. Normal human urine and saliva samples tested negative for this kit.

Cell culture supernatant, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.

CROSS REACTIVITY

Human alpha 1 Anti-chymotrypsin protein and human PAI protein were prepared at 2,000 ng/mL and 1,000 ng/mL respectively and assayed for cross reactivity. No cross-reactivity was observed.

INTERFERENCE

Human alpha 1 Anti-chymotrypsin protein and human PAI protein were prepared at 2,000 ng/mL and 1,000 ng/mL respectively and assayed for and tested for interference. No interference with was observed.

SPECIES REACTIVITY

This kit recognizes human alpha 1 Antitrypsin protein.
Other species reactivity was determined by measuring alpha 1 Antitrypsin (1:10,000) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Hamster, Guinea Pig, Rabbit, Dog, Goat, Pig and Cow.

CALIBRATION

This immunoassay is calibrated against a highly purified human alpha 1 Antitrypsin. The NIBSC/WHO unclassified purified human alpha 1 Antitrypsin preparation 05/162 was evaluated in this kit.

The dose response curve of the unclassified standard alpha 1 Antitrypsin parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human alpha 1 Antitrypsin kit to approximate NIBSC 05/162 units, use the equation below.

NIBSC (05/162) approximate value (ng/mL) = 0.3649 x SimpleStep Human alpha 1 Antitrypsin value (ng/mL).

alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it's main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.