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Human ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ALT in Cell culture media, Citrate plasma, Serum, EDTA Plasma samples.

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

109.38 - 7000 pg/mL

Assay time

1h 30m

Sensitivity

= 47.34 pg/mL

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Shipping To:
Korea, Rep of

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

1 x 384 Tests
Components
10X Human ALT Capture Antibody
1 x 600 µL
10X Human ALT Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
50X Cell Extraction Enhancer Solution (ab193971)
1 x 5 mL
Antibody Diluent 4BI
1 x 6 mL
Human ALT Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

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Human ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ALT in Cell culture media, Citrate plasma, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

109.38 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 47.34 pg/mL

Precision

Intra assay

Sample
Serum
n
8
mean
-
SD
-
C.V.
4.5

Recovery

Sample specific recovery

Sample type
Cell culture media
Average %
= 95
Range
Sample type
Citrate plasma
Average %
= 92
Range
Sample type
EDTA Plasma
Average %
= 111
Range
Sample type
Serum
Average %
= 117
Range

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human ALT ELISA Kit (ab234578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALT protein in cell culture media, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALT with 47.34 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only.

Tissue extract samples have not been tested with this kit.

This kit is incompatible with plasma- heparin and cell extract samples.


SPECIES REACTIVITY This kit recognizes human ALT protein. Other species reactivity not determined. Please contact our Technical Support team for more information.

ALT (Alanine aminotransferase 1, ALT1) is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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