Human ANGPTL3 ELISA Kit

Human ANGPTL3 ELISA Kit (ab254510) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ANGPTL3 protein in cell culture media, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ANGPTL3 with 103.25 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ANGPTL3 (Angiopoietin-like 3) is a glycoprotein made in the liver and involved in lipid metabolism. ANGPTL3 can inhibit endothelial lipase and ANGPTL3 levels correlate with plasma HDL cholesterol, implicating it in modulation of HDL levels. ANGPTL3 expression is also associated with triglyceride levels, as loss of function mutations in ANGPTL3 is often found in individuals with low plasma triglyceride levels. Additionally, interference of ANGPTL3 interactions with LPL (the enzyme that hydrolyzes circulating triglycerides) in mice resulted in a reduction of serum triglyceride levels.