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Human Apolipoprotein AII ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Apolipoprotein AII in Urine, Plasma, Cell culture supernatant, Serum samples.

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Sensitivity

= 6 pg/mL

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Shipping To:
Korea, Rep of

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

10 x 96 Tests
Components
10X Human Apolipoprotein AII Capture Antibody
1 x 6000 µL
10X Human Apolipoprotein AII Detector Antibody
1 x 6000 µL
10X Wash Buffer PT (ab206977)
1 x 200 mL
Antibody Diluent 5BI
10 x 6 mL
Human Apolipoprotein AII Lyophilized Purified Protein
2 x 10 Vial
Plate Seals
10 x 1 Unit
Sample Diluent NS (ab193972)
2 x 250 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
10 x 1 Unit
Stop Solution
1 x 120 mL
TMB Development Solution
1 x 120 mL

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Human Apolipoprotein AII ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Apolipoprotein AII in Urine, Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 6 pg/mL

Precision

Intra assay

Sample
HeLa Extract
n
5
mean
-
SD
-
C.V.
2

Inter assay

Sample
HeLa Extract
n
3
mean
-
SD
-
C.V.
10.6

Recovery

Sample specific recovery

Sample type
Urine
Average %
= 90.2
Range
89.3 - 90.8 %
Sample type
Cell culture media
Average %
= 125.2
Range
122.2 - 127.8 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Apolipoprotein A II ELISA kit (ab184859) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein A II protein in human serum, plasma, urine, cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Apolipoprotein A II with 6 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Apolipoprotein AII may stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. ApoA2 forms a disulfide-linked homodimer. Apolipoprotein AII also forms a disulfide-linked heterodimer with APOD and interacts with HCV core protein, APOA1BP and NDRG1.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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