Human CRP ELISA Kit (C-Reactive protein)

Human CRP ELISA Kit (C-Reactive protein) (ab260058) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CRP (C-Reactive protein) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CRP (C-Reactive protein) with 5.36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY: This kit recognizes both native and recombinant human and monkey CRP protein in serum, plasma, and cell culture supernatant.

Cell and tissue extract, urine, milk, and saliva samples have not been tested with this kit.

CROSS REACTIVITY: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and assayed for cross reactivity. No cross-reactivity was observed.

INTERFERENCE: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and tested for interference. No interference with was observed.

SPECIES REACTIVITY: This kit recognizes human and monkey CRP protein.

Other species reactivity was determined by measuring 1:1000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.

Reactivity < 3% was determined for the following species: Mouse, Rat, Cow.

Other species reactivity not determined.

C-reactive protein (CRP) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6. This kit requires the use of acid treatment for the disassociation of CRP.