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Human Factor IX/PTC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor IX/PTC in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
Alternative names=Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9
- Detection method
Colorimetric
- Sample types
Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
97.656 - 6250 pg/mL
- Assay time
1h 30m
- Sensitivity
= 15.884 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human Factor IX/PTC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Factor IX/PTC in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.
Alternative names=Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9
Key facts
- Detection method
Colorimetric
- Sample types
Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
97.656 - 6250 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 15.884 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 7.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 10.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Citrate plasma | Average % = 116 | Range 111 - 119 % |
Sample type EDTA Plasma | Average % = 106 | Range 95 - 115 % |
Sample type Heparin Plasma | Average % = 112 | Range 103 - 118 % |
Sample type Serum | Average % = 105 | Range 92 - 113 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Factor IX/PTC SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor IX/PTC protein in human serum and plasma. Quantitate Human Factor IX/PTC with 15.884 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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