Human Factor XII ELISA Kit

As of April 2020, Human Factor XII ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.

Human Factor XII ELISA Kit (ab192144) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor XII protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor XII with 0.1 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Factor XII is a 80 kDa plasma serine protease involved in the coagulation cascade. Factor XII is activated by proteolysis and the product, Factor XIIa, goes on to activate Factor XI and prekallikrein in in vitro models of blood cogulation. Its involvement in the beginning of the coagulation cascade is only seen in in vitro studies, and is thought to have no effect on coagulation initiation in vivo. While Factor XII deficiency in humans is asymptomatic, Factor XII plays a role in later stages of clot formation. Deficiency of Factor XII affects the in vitro measurements of partial thromboplastin time in human blood, and its absence decreases the susceptibility of mice to thrombosis.