Human Growth Hormone ELISA Kit

Human Growth Hormone ELISA Kit (ab190811) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Growth Hormone protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Growth Hormone with 1.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human Growth Hormone protein in serum, plasma, milk, urine, and cell culture supernatant samples only.

Saliva, and cell and tissue extract samples have not been tested with this kit.

SPECIES REACTIVITY

This kit recognizes human Growth Hormone protein.

Other species reactivity was determined by measuring neat serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.

Reactivity < 3% was determined for the following species:

• Mouse


• Rat


• Cow

CALIBRATION

This immunoassay is calibrated against a highly purified human Growth Hormone. The NIBSC/WHO unclassified purified human Growth Hormone preparation 80/505 was evaluated in this kit.

The dose response curve of the unclassified standard Growth Hormone parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human Growth Hormone kit to approximate NIBSC 80/505 units, use the equation below.

NIBSC (80/505) approximate value (mIU/mL) = 2.3x10^-6 x SimpleStep Human Growth Hormone value (pg/mL).

Growth Hormone (also known as GH, GH1, Somatotropin and Pituitary growth hormone) is a circulations hormone that plays an important role in somatic growth control. Growth Hormone binds to the Growth Hormone Receptor present in a variety of tissues and induces signaling cascades. Growth Hormone secretion is controlled positively and negatively by other hormones, including Ghrelin and Somatostatin. Overproduction of Growth Hormone can result in gigantism whereas deficiency can contribute to dwarfism.