Human IgE ELISA Kit

Human IgE ELISA Kit (ab195216) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgE protein in serum, cell culture supernatant, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IgE with 0.02 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Immunoglobulin E (IgE) is a class of antibodies that plays a fundamental role in various allergic reactions. IgE is composed of two heavy chains and two light chains, found to be typical of Ig molecules. The epsilon heavy chain attaches to its high-affinity receptor Fc epsilon receptor (FcƐRI) found primarily on mast cells, basophils, and eosinophils. Binding of IgE to its receptor initiates a release of vasoactive amines (i.e. histamines) and other mediators of allergic responses into the surrounding tissue. Human IgE is produced in the lungs, skin and mucous membrane, but is typically the least abundant antibody isotope. Its main function is immunity against parasites such as parasitic worms like *Schistosoma mansoni, Trichinella spiralis*, and *Fasciola hepatica*. IgE also plays a role in type I hypersensitivity, which manifests various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergy, and some types of chronic urticaria, and atopic dermatitis.