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Human Interferon gamma ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Interferon gamma in Cell culture media, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum samples.

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Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

7.813 - 500 pg/mL

Assay time

1h 30m

Sensitivity

= 3.428 pg/mL

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Shipping To:
Korea, Rep of

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

1 x 384 Tests
Components
10X Human Interferon gamma Capture Antibody
1 x 600 µL
10X Human Interferon gamma Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
Antibody Diluent 4BI
1 x 6 mL
Human Interferon gamma Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

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Human Interferon gamma ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Interferon gamma in Cell culture media, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

7.813 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 3.428 pg/mL

Precision

Intra assay

Sample
Supernatant
n
8
mean
-
SD
-
C.V.
7.4

Inter assay

Sample
Supernatant
n
3
mean
-
SD
-
C.V.
11.38

Recovery

Sample specific recovery

Sample type
Cell culture supernatant
Average %
= 104
Range
97 - 115 %
Sample type
Citrate plasma
Average %
= 101
Range
95 - 109 %
Sample type
EDTA Plasma
Average %
= 100
Range
93 - 106 %
Sample type
Heparin Plasma
Average %
= 103
Range
93 - 114 %
Sample type
Serum
Average %
= 101
Range
96 - 104 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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