Human Leptin ELISA Kit

As of January 16, 2020, Human Leptin ELISA kit (ab179884) has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.

Human Leptin ELISA kit (ab179884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Leptin protein in human serum, plasma and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Leptin with 4.65 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause in obesity.Acute low levels of leptin triggers increased appetite and chronic leptin deficiency can block the onset of puberty.