Human MCP-1 ELISA Kit

Human MCP1 (CCL2) ELISA kit (ab179886) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in human serum, plasma, urine, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human MCP1 with 1.26 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

ASSAY SPECIFICITY This kit recognizes both native and recombinant human MCP1 protein in serum, plasma, urine, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

SPECIES REACTIVITY This kit recognizes human MCP1 protein.
Other species reactivity was determined by measuring MCP1 (100%) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse and cow.

CROSS REACTIVITY Recombinant mouse MCP1 and rat MCP1 were prepared at 100 pg/mL and 50 pg/mL and assayed for cross reactivity. Cross-reactivity was only observed at 50 ng/mL.

CALIBRATION

This immunoassay is calibrated against a highly purified human MCP1. The NIBSC/WHO unclassified purified human MCP1 preparation 92/794 was evaluated in this kit.

The dose response curve of the unclassified standard MCP1 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep human MCP1 kit to approximate NIBSC 92/794 units, use the equation below.

NIBSC (92/794) approximate value (U/mL) = 0.000266 x SimpleStep human MCP1 value (pg/mL).

MCP-1 (CCL2) is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.