Human PSA ELISA Kit

Human PSA ELISA Kit (ab264615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSA protein in edta plasma, hep plasma, serum, fetal bovine serum, urine, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PSA with 8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Prostate specific antigen (PSA) or also commonly termed tissue Kallikrein-3 (KLK3) is a 34 kD serine protease from the Kallikrein family. PSA has very high expression in the prostate gland, but expression also has been reported in the breast and saliva. PSA functions by hydrolyzing semenogelin-1 leading to the liquefaction of the seminal coagulum. This is essential for the proper release of sperm during ejaculation. The majority (>70%) of PSA is known to form a complex with alpha-1-antichymotrypsin (PSA-ACT) in the blood. The remaining PSA exists as an unbound inactive enzyme known as Free PSA. PSA is best characterized as a clinical biomarker for Prostate Cancer. During prostate cancer progression, excess amounts of PSA are leaked into the blood dramatically elevating both Total and Free PSA levels. This assay detects both total and free PSA levels.