Human SFRP1 ELISA Kit

Human SFRP1 ELISA kit (ab277082) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SFRP1 protein in human serum and plasma - heparin. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SFRP1 with 18.5 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Soluble frizzled-related protein 1 (SFRP1) is part of a family of SFRPs that function as modulators of Wnt signaling through direct interaction with Wnts. SFRPs have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels, has antiproliferative effects on vascular cells and can induce in vivo, an angiogenic response. In vascular cell cycle, SFRP1 delays the G1 phase and entry into the S phase. In kidney development, it inhibits tubule formation and bud growth in metanephroi. Additionally, it Inhibits WNT1/WNT4-mediated TCF-dependent transcription. SFRP1 shares 96.8%, 95.8%, 97.8%, and 98.2% sequence homology with mouse, rat, dog, and bovine, respectively.